The ESTIV Members AreaThe programme as of 1.8. 2025 – the programme is still subject of minor changes
| e/Day | Sunday November 16, 2025 | Monday November 17, 2025 | Tuesday November 18, 2025 | Wednesday November 19, 2025 | Thursday November 20, 2025 | Friday November 21, 2025 |
| 8:30-9:30 | General in vitro cytotoxicity and cell death assays – Mathieu Vinken, Vrije Universiteit Brussel, Belgium | Alternative methods and OECD testing guidelines – Helena Kandárová, CEM, Slovak Academy of Sciences, Slovakia | In vitro methods for skin irritation and corrosion testing – Helena Kandárová, CEM, Slovak Academy of Sciences, Slovakia | In vitro kinetics and in vitro-in vivo extrapolation. Bas Blaauboer, Utrecht University, The Netherlands | In vitro methods for testing genetic toxicity and carcinogenicity – Philippe Vanparys, GENTOXICON, Belgium | |
| 9:30-10:30 | Introduction to adverse outcome pathways in in vitro toxicology. Mathieu Vinken, Vrije Universiteit Brussel, Belgium | Developmental Neurotoxicity (DNT): Concepts, Strategies, and Future Directions Eliska Kuchovska, IUF – IUF – Leibniz Research Institute for Environmental Medicine, Germany | In vitro methods for eye hazard testing – Helena Kandárová, CEM, Slovak Academy of Sciences, Slovakia | PBK modelling and quantitative in vitro-in vivo extrapolation (QIVIVE). Hsing-Chieh Lin, esqLabs | Group exercise | |
| 10:30-11:00 | Break | Break | Break | Break | Break | |
| 11:00-12:00 | Good in vitro method practice (GIVIMP and OECD GLP) Helena Kandárová, CEM, Slovak Academy of Sciences, Slovakia | In vitro methods for testing immunotoxicity and skin sensitisation – Sandra Coecke, JRC, Italy | Pitfalls of in vitro testing. Lucia Račková, CEM, Slovak Academy of Sciences, Slovakia | Development and implementation of new approach methodologies in genetic toxicology. Inger Brandsma, Toxys, The Netherlands | Group exercise | |
| 12:00-13:00 | Lunch Break | Lunch Break | Lunch Break | Lunch Break | ||
| 13:00-14:00 | Liver-based in vitro models for toxicology purposes – Mathieu Vinken, Vrije Universiteit Brussel, Belgium | Animal-product free cell culture: status and challenges. Arno Gutleb, LIST, Luxembourg | The use of ‘omics’ in in vitro toxicology – Theo de Kok, Maastricht University, The Netherlands | In vitro toxicology testing of nanomaterials. Arno Gutleb, LIST, Luxembourg | Group exercise | |
| 14:00-15:00 | Lung-based in vitro models for toxicology purposes – Arno Gutleb, Luxembourg Institute of Science and Technology, Luxembourg | Gastro-intestinal in vitro models for toxicology purposes – Marek Puskar/Jan Markus MatTek Life Sciences/BICO group Slovakia Slovakia | Data management for in vitro toxicology. Luiz Ladeira, GIGA Institute – Molecular and Computational Biology, University of Liège, Belgium | Concepts in microfluidics. Silvia Quaglio, IVTech, Italy | Group exercise | |
| 15:00-15:30 | Break | Break | Break | |||
| 15:30 – 17:30 | Registration starts from 15:30-16:00 Location: Hotel Park Inn, Rybne Namestie 1, 81102 Bratislava | Hands-on training I 3D-model assembly and Vitrocell systems for inhalation toxicity testing. Peter Pôbiš, IEPT CEM, Slovakia | Hands-on training II In vitro gastro-intestinal toxicity testing with 3D models – Marek Puškár, MatTek Life Sciences/BICO group Slovakia Slovakia | Hands-on training III In vitro eye irritation testing with 3D cornea models – Marek Puškár, Silvia Letšiová, MatTek Life Sciences/BICO group Slovakia | Hands-on training IV Introduction into the microfluidic work. Tommaso Sbrana, IVTech, Italy | Written Exam |
| 16:00-17:30 Course introduction Helena Kandárová, CEM, Slovak Academy of Sciences, Slovakia ESTIV-ECN introduction Luiz Ladeira, GIGA Institute – Molecular and Computational Biology, University of Liège, Belgium Participants Introduction and refreshments | Guided tour – Bratislava old town: 18:30 – 20:00 (start in front of Park Inn) Social event at 20:00 In the spirit of traditional Slovak cuisine (vegetarian and vegan options included) Location: Koliba Kamzík, Michalská 16 Bratislava | End of the course Adjourn |
Hands-on training V
Introduction into the Human on the Chip/Organ on a chip work ,
Hands-on training VI – 3D printing introduction and demonstration,
Hands-on training V
Introduction into the Human on the Chip/Organ on a chip work